protran 0 2 nitrocellulose membrane (Amersham Life Sciences Inc)

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Amersham Life Sciences Inc protran 0 2 nitrocellulose membrane
Protran 0 2 Nitrocellulose Membrane, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nitrocellulose+membranes/membrane+nitrocellulose/pmc13202259-68-1-0
Average 86 stars, based on 1 article reviews

protran 0 2 nitrocellulose membrane - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Heat treatment combined with hybrid exercises retraining mitigates cellular markers of protein turnover after hindlimb suspension in male mice: A pilot study
Article Snippet: Subsequently, 50 μg of protein was mixed with Laemmli sample buffer, separated by SDS–PAGE, and transferred onto nitrocellulose membranes (Amersham, Protran Premium, 0.2 μm; Cytiva, Marlborough, MA, USA).

Article Title: RPRD1A drives lenvatinib resistance in hepatocellular carcinoma via the ITGA5-FAK signaling axis
Article Snippet: Proteins were transferred to nitrocellulose membranes (0.2 μm, Amersham).

Article Title: Inhibition of high risk HPV31 E8^E2 repressor activity enables differentiation-independent genome amplification and E4 expression.
Article Snippet: Proteins were blotted onto 0.22 μm nitrocellulose membranes (Amersham).

Article Title: A mechanism for substrate quality control in outer membrane protein assembly.
Article Snippet: Proteins were transferred onto nitrocellulose membranes (Amersham Protran; Cytiva) using a semidry transfer system (Trans-Blot T urbo; Bio-R ad).

Article Title: 6mA DNA methylation dynamics during chloroplast development in Agave angustifolia Haw.
Article Snippet: Main Conclusion N6-methyladenine (6mA) DNA accumulates during chloroplast maturation in Agave angustifolia Haw. and covaries with METTL4 protein abundance, identifying 6mA as a candidate epigenetic mark associated with plastid biogenesis.. Abstract Chloroplast development requires precise coordination of nuclear and organellar genomes, yet the epigenetic mechanisms regulating this process remain poorly understood.. Here, we provide the first description of N6-methyladenine (6mA), a dynamic DNA modification, during Agave angustifolia Haw. chloroplast biogenesis.

Nucleic Acid Electrophoresis:

Article Title: S100A10-ANXA2 tetramer inhibition hampers hepatic stellate cell activation in human MASLD organoids.
Article Snippet: Protein 14 EMBO Molecular Medicine © The Author(s) concentration was measured using BCA protein assay kit (Pierce Biotechnology, 23225). .. The extracted proteins (5–10 μg) were separated by 5-20% gradient sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Amersham, RPN303D). .. The membranes were blocked with 5% non-fat dry milk in TBS-T (0.1% Tween-20) and incubated overnight at 4 °C with the S100A10 (1/2000, Abcam, ab76472), AnnexinA2 (1/2000, Cell Signalling, 8235S), pSTAT3 (1/1000, Cell Signalling, 9145), STAT3 (1/1000, Santa Cruz, sc482), pSMAD2 (1/1000, GeneTex, GTX133614), SMAD2/3 (1/10000, GeneTex, GTX111123), pSMAD1/5/9 (1/1000, Cell Signaling, 13820), SMAD1 (1/1000, Cell Signaling, 9743), ADRP (1/1000, Progen, 610102), ERM ((Ezrin/Radixin/Moesin), 1/ 1000, Cell Signaling, 3142), Pro-caspase 3 (1/1000, Cell Signaling, 14220), Cleaved-caspase 3 (1/1000, Cell Signaling, 9664), Tubulin (1/5000, Cell Signaling, 2128) and α-SMA (kind gift from Prof. Marie-Luce Bochaton-Piallat, University of Geneva).

SDS Page:

Article Title: Nectin receptors mediate mechanosensing to YAP via AKT.
Article Snippet: Lysates were mixed with LDS Sample Buffer 4x (NP0007, Thermo Fisher Scientific) and 5% (v/v) 2-mercaptoethanol (M3148, Merck), heated to 95°C for 5 min and stored at -20°C. .. Lysates were separated by SDS-PAGE using 1.5-mm Novex gel casting cassettes followed by electrophoretic transfer to 0.45-μm nitrocellulose membranes (10600020, Amersham). .. Membranes were washed in Tris-buffered saline (24025, Severn Biotech Ltd) containing 0.05% (v/v) Tween-20 (P1379, Merck, TBS-T) and AR TI CL E IN P RE SS blocked with 5% (w/v) dried skimmed milk powder (84615.0500, Avantor) in TBS-T for 30 min (milk TBS-T).

Staining:

Article Title: Zip14 (Slc39a14) metal transporter dysfunction leads to Akkermansia dysbiosis and inflammation in intestinal epithelium via epigenetic changes.
Article Snippet: Protein concentration was measured using the Pierce BCA Protein Assay (ThermoFisher). .. Proteins were separated using 10% mini-PROTEAN TGX gels (BioRad) and transferred to 0.45 m nitrocellulose membranes (Amersham) for 1 h at 60 V. Ponceau Red staining verified transfer uniformity. .. Using the PageRuler Plus Prestained Protein Ladder (ThermoFisher), membranes were sectioned as needed and probed with antibodies.

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